Western Blot:Article Title: Cancer-associated fibroblast-derived extracellular vesicles deliver ATP6V1C1 to promote lung adenocarcinoma metastasis via the ID1-IGFBP3 axis.
Article Snippet: .. Data are mean ± SD (n = 20). (D) Western blot of ATP6V1C1, histone H3 (nuclear marker), and α-tubulin (cytoplasmic marker) in subcellular fractions from OE.ATP6V1C1-EGFP-PDCs. (E) Subcellular localization pattern of ATP6V1C1 from the Human Protein Atlas. (F) ChIP-seq heatmaps for all genes and peak profiles of ATP6V1C1 binding relative to transcription start/end sites (TSS/TES). (G) Correlation analysis of ATP6V1C1, ID1, and IGFBP3 expression in the TCGA-LUAD cohort. (H) Dual-luciferase reporter assay demonstrating ATP6V1C1-mediated repression of the ID1 enhancer. (I) Western blot of ATP6V1C1, IGFBP3, and ID1 in A549 cells after ATP6V1C1 silencing. (J, K, L) Migration assays and quantification of LUAD-PDCs under the indicated conditions. ..
Marker:Article Title: Cancer-associated fibroblast-derived extracellular vesicles deliver ATP6V1C1 to promote lung adenocarcinoma metastasis via the ID1-IGFBP3 axis.
Article Snippet: .. Data are mean ± SD (n = 20). (D) Western blot of ATP6V1C1, histone H3 (nuclear marker), and α-tubulin (cytoplasmic marker) in subcellular fractions from OE.ATP6V1C1-EGFP-PDCs. (E) Subcellular localization pattern of ATP6V1C1 from the Human Protein Atlas. (F) ChIP-seq heatmaps for all genes and peak profiles of ATP6V1C1 binding relative to transcription start/end sites (TSS/TES). (G) Correlation analysis of ATP6V1C1, ID1, and IGFBP3 expression in the TCGA-LUAD cohort. (H) Dual-luciferase reporter assay demonstrating ATP6V1C1-mediated repression of the ID1 enhancer. (I) Western blot of ATP6V1C1, IGFBP3, and ID1 in A549 cells after ATP6V1C1 silencing. (J, K, L) Migration assays and quantification of LUAD-PDCs under the indicated conditions. ..
Chromatin Immunoprecipitation:Article Title: Cancer-associated fibroblast-derived extracellular vesicles deliver ATP6V1C1 to promote lung adenocarcinoma metastasis via the ID1-IGFBP3 axis.
Article Snippet: .. Data are mean ± SD (n = 20). (D) Western blot of ATP6V1C1, histone H3 (nuclear marker), and α-tubulin (cytoplasmic marker) in subcellular fractions from OE.ATP6V1C1-EGFP-PDCs. (E) Subcellular localization pattern of ATP6V1C1 from the Human Protein Atlas. (F) ChIP-seq heatmaps for all genes and peak profiles of ATP6V1C1 binding relative to transcription start/end sites (TSS/TES). (G) Correlation analysis of ATP6V1C1, ID1, and IGFBP3 expression in the TCGA-LUAD cohort. (H) Dual-luciferase reporter assay demonstrating ATP6V1C1-mediated repression of the ID1 enhancer. (I) Western blot of ATP6V1C1, IGFBP3, and ID1 in A549 cells after ATP6V1C1 silencing. (J, K, L) Migration assays and quantification of LUAD-PDCs under the indicated conditions. ..
Binding Assay:Article Title: Cancer-associated fibroblast-derived extracellular vesicles deliver ATP6V1C1 to promote lung adenocarcinoma metastasis via the ID1-IGFBP3 axis.
Article Snippet: .. Data are mean ± SD (n = 20). (D) Western blot of ATP6V1C1, histone H3 (nuclear marker), and α-tubulin (cytoplasmic marker) in subcellular fractions from OE.ATP6V1C1-EGFP-PDCs. (E) Subcellular localization pattern of ATP6V1C1 from the Human Protein Atlas. (F) ChIP-seq heatmaps for all genes and peak profiles of ATP6V1C1 binding relative to transcription start/end sites (TSS/TES). (G) Correlation analysis of ATP6V1C1, ID1, and IGFBP3 expression in the TCGA-LUAD cohort. (H) Dual-luciferase reporter assay demonstrating ATP6V1C1-mediated repression of the ID1 enhancer. (I) Western blot of ATP6V1C1, IGFBP3, and ID1 in A549 cells after ATP6V1C1 silencing. (J, K, L) Migration assays and quantification of LUAD-PDCs under the indicated conditions. ..
Expressing:Article Title: Cancer-associated fibroblast-derived extracellular vesicles deliver ATP6V1C1 to promote lung adenocarcinoma metastasis via the ID1-IGFBP3 axis.
Article Snippet: .. Data are mean ± SD (n = 20). (D) Western blot of ATP6V1C1, histone H3 (nuclear marker), and α-tubulin (cytoplasmic marker) in subcellular fractions from OE.ATP6V1C1-EGFP-PDCs. (E) Subcellular localization pattern of ATP6V1C1 from the Human Protein Atlas. (F) ChIP-seq heatmaps for all genes and peak profiles of ATP6V1C1 binding relative to transcription start/end sites (TSS/TES). (G) Correlation analysis of ATP6V1C1, ID1, and IGFBP3 expression in the TCGA-LUAD cohort. (H) Dual-luciferase reporter assay demonstrating ATP6V1C1-mediated repression of the ID1 enhancer. (I) Western blot of ATP6V1C1, IGFBP3, and ID1 in A549 cells after ATP6V1C1 silencing. (J, K, L) Migration assays and quantification of LUAD-PDCs under the indicated conditions. ..
Reporter Assay:Article Title: Cancer-associated fibroblast-derived extracellular vesicles deliver ATP6V1C1 to promote lung adenocarcinoma metastasis via the ID1-IGFBP3 axis.
Article Snippet: .. Data are mean ± SD (n = 20). (D) Western blot of ATP6V1C1, histone H3 (nuclear marker), and α-tubulin (cytoplasmic marker) in subcellular fractions from OE.ATP6V1C1-EGFP-PDCs. (E) Subcellular localization pattern of ATP6V1C1 from the Human Protein Atlas. (F) ChIP-seq heatmaps for all genes and peak profiles of ATP6V1C1 binding relative to transcription start/end sites (TSS/TES). (G) Correlation analysis of ATP6V1C1, ID1, and IGFBP3 expression in the TCGA-LUAD cohort. (H) Dual-luciferase reporter assay demonstrating ATP6V1C1-mediated repression of the ID1 enhancer. (I) Western blot of ATP6V1C1, IGFBP3, and ID1 in A549 cells after ATP6V1C1 silencing. (J, K, L) Migration assays and quantification of LUAD-PDCs under the indicated conditions. ..
Migration:Article Title: Cancer-associated fibroblast-derived extracellular vesicles deliver ATP6V1C1 to promote lung adenocarcinoma metastasis via the ID1-IGFBP3 axis.
Article Snippet: .. Data are mean ± SD (n = 20). (D) Western blot of ATP6V1C1, histone H3 (nuclear marker), and α-tubulin (cytoplasmic marker) in subcellular fractions from OE.ATP6V1C1-EGFP-PDCs. (E) Subcellular localization pattern of ATP6V1C1 from the Human Protein Atlas. (F) ChIP-seq heatmaps for all genes and peak profiles of ATP6V1C1 binding relative to transcription start/end sites (TSS/TES). (G) Correlation analysis of ATP6V1C1, ID1, and IGFBP3 expression in the TCGA-LUAD cohort. (H) Dual-luciferase reporter assay demonstrating ATP6V1C1-mediated repression of the ID1 enhancer. (I) Western blot of ATP6V1C1, IGFBP3, and ID1 in A549 cells after ATP6V1C1 silencing. (J, K, L) Migration assays and quantification of LUAD-PDCs under the indicated conditions. ..
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